Nutrition and factors limiting the growth of a methanogenic bacterium (Methanobacterium thermoautotrophicum) |
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Authors: | Geoffrey T. Taylor S. John Pirt |
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Affiliation: | (1) Microbiology Department, Queen Elizabeth College, Campden Hill Road, London W.8, England;(2) Present address: Brewing Research Foundation, Nr Redhill, Nutfield, Surrey, England |
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Abstract: | The purification of Methanobacterium thermoautotrophicum from a culture contaminated with a heterotrophic organism is described. A defined inorganic medium under H2/CO2 (80:20 v/v) has been developed to support growth of M. thermoautotrophicum up to a concentration of at least 1.7 g dry weight/l. In a conventional medium iron and nitrogen sources were found to be growth-limiting factors. Throughout most of the culture period the rate of transfer of hydrogen or carbon dioxide from gas to liquid was the factor which controlled the growth rate.The growth yields of bacteria were in the range 0.6–1.6 g dry weight/mole CH4.Abbreviation TGP thioglycollate peptone medium |
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Keywords: | Purification Growth-limiting factors Growth yields Methanobacterium thermoautotrophicum |
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