Electron microscopic visualization of the arrangement of the two protein components of (Na + K)-ATPase |
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Authors: | Frank Vogel Helmut W Meyer Richard Grosse Kurt RH Repke |
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Institution: | 1. Department of Electron Microscopy, Academy of Sciences of GDR, 1115 Berlin-Buch, Germany;2. Biomembrane Section in Central Institute of Molecular Biology, Academy of Sciences of GDR, 1115 Berlin-Buch, Germany;3. Department of Electron Microscopy of Friedrich-Schiller University, 69 Jena, G.D.R. |
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Abstract: | The information obtained by electron microscopic examination of highly purified membrane preparations of (Na+ + K+)-ATPase after freeze-fracturing or negative staining suggests the following conclusions. The catalytic 100 000 dalton protein component penetrates with its greater ‘globular’ mass the plasma membrane and protudes with its smaller mass from the protoplasmic surface by a stalked knob carrying the catalytic centre. The 40 000 dalton glycoprotein component is anchored in the membrane interior by a non-polar ‘fibrous’ side chain, whereas its major polar mass projects from the outer membrane surface forming a surface coat of ill-definable substructure. |
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