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An isolation procedure for arachidonic acid producing Mortierella species |
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| Authors: | Alfred Botha Ida Paul Cecilia Roux Johan L.F. Kock Dennis J. Coetzee Tersia Strauss Charlotte Maree |
| Affiliation: | (1) Department of Microbiology and Biochemistry, University of the Orange Free State, P.O. Box 339, Bloemfontein, 9301, South Africa;(2) National Collection of Fungi, Biosystematics Division, Plant Protection Research Institute, Pretoria, South Africa;(3) Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland, 7602, South Africa |
| Abstract: | Malt extract agar and an incubation temperature of 5 °C were used to selectively isolate representatives of the genus Mortierella from soil. Fungi in a soil sample from mountain grassland able to grow under these conditions, amounted to a total of 2640 colony forming units per gram soil. Circa 94% of the total fungal isolates represented Mortierella subgenus Mortierella. The rest of the colony-forming units consisted of Mucor isolates (6.0%) and higher fungi (1.5%). All the Mortierella isolates produced arachidonic acid. |
| Keywords: | Arachidonic acid isolation Mortierella soil |
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