芒果炭疽病菌β-微管蛋白基因的克隆及其与多菌灵抗药性发生的关系

参照豆科合萌属 (Aeschynomene)作物炭疽病菌的tub1和tub2基因序列设计了 2对引物 ,分别从芒果 (Man gifera)炭疽病菌对多菌灵 (MBC)田间抗药性 (MBCR)和敏感 (MBCS)的菌株中扩增 β_微管蛋白基因。结果只有以tub2为参照设计的引物扩增到了特异片段。进一步对全基因进行了克隆和测序。该基因序列全长 1344bp ,编码4 4 7aa ,其核苷酸和氨基酸序列与豆科合萌属炭疽病菌的tub2基因高度同源。对芒果炭疽病菌抗、感菌株 β_微管蛋白氨基酸序列进行比较分析 ,发现第 181、2 37和 36 3位氨基酸发生了突变 ,而其它位置 (如第 198位或 2 0 0位 )均不变

Cloning of-Tubulin Gene and Their Correlation with Conferred Carbendazim Resistance of Colletotrichum gloeosporioides Penz in Mango

The total DNA isolated from MBC-resistant and MBC-sensitive isolates of Colletotrichum gloeosporioides Penz (C.g.M) of mango were used as templates in PCR amplification using consensus oligo nucleotide primers designed according to the known sequence data of beta-tubulin-encoding gene (tub 1 and tub2) of Colletotrichum gloeosporioides f. sp. aeschynomene (C. g. A). Only the primers designed according to C. g. A tub2 amplified specific fragments. These amplified fragments were cloned and sequenced. The results showed that these fragments have 1344bp and deduced 447 amino acid, which were highly homologous to C.g.A tub2. MBC-resistant isolates did not carry the allelic mutation at amino acid codes 198 and 200 of beta-tubulin gene in comparison with the sensitive isolates. However, the amino acid altered in codes 181, 237 and 363.