Isolation of the phe-operon from G. stearothermophilus comprising the phenol degradative meta-pathway genes and a novel transcriptional regulator |
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| Authors: | Bastian Omokoko Uwe K Jäntges Martin Zimmermann Monika Reiss Winfried Hartmeier |
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| Affiliation: | (1) Department of Biotechnology, RWTH Aachen University, Worringer Weg 1, 52074 Aachen, Germany;(2) Department of Biology IV (Microbiology & Genetics), RWTH Aachen University, Worringer Weg 1, 52074 Aachen, Germany |
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| Abstract: | Background Geobacillus stearothermophilus is able to utilize phenol as a sole carbon source. A DNA fragment encoding a phenol hydroxylase catalyzing the first step in the meta-pathway has been isolated previously. Based on these findings a PCR-based DNA walk was performed initially to isolate a catechol 2,3-dioxygenase for biosensoric applications but was continued to elucidate the organisation of the genes encoding the proteins for the metabolization of phenol. |
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