|
|||||
|
|
The beta-tubulin genes of two Strongyloides species |
|
| Authors: | Melville Lewis A Sykes Alex M McCarthy James S |
| Institution: | Clinical Tropical Medicine Laboratory, Queensland Institute of Medical Research, University of Queensland, Herston, QLD 4006, Australia. |
| Abstract: | The World Health Organization is sponsoring major treatment programs with the aim of controlling helminth infection throughout the tropical world. Prominent among the anthelmintics recommended for use in these programs are drugs in the benzimidazole (BZ) class. Resistance to these drugs has been associated with polymorphisms in the beta-tubulin gene. We have cloned and sequenced the beta-tubulin genes of Strongyloides stercoralis and Strongyloides ratti and have proceeded to develop a protocol for genotyping single worms for polymorphisms in beta-tubulin. Our findings indicate that S. ratti has a single beta-tubulin gene, making DNA sequence analysis of a single larva PCR product a feasible means of studying BZ resistance in these species. Our genotyping test allows the identification of polymorphisms at codons 167, 198, and 200 in the Strongyloides beta-tubulin gene, thus enabling survey for BZ resistant genotypes. |
| Keywords: | Strongyloides stercoralis Strongyloides ratti Albendazole Thiabendazole Benzimidazole drugs Strongyloidiasis β-Tubulin gene BZ benzimidazole WHO World Health Organization DNA deoxyribonucleic acid cDNA complementary deoxyribonucleic acid RNA ribonucleic acid mRNA messenger ribonucleic acid RACE rapid amplification of complementary deoxyribonucleic acid ends Phe phenylalanine Tyr tyrosine PCR polymerase chain reaction EST expressed sequence tag bp base pair kb kilobase SDS sodium dodecyl sulphate SSC 0 15 M sodium chloride 0 015 M sodium citrate NCBI National Centre for Biotechnology Information CTAB cetyl-trimethyl ammonium bromide dUTP deoxyuracil-triphosphate |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |