Transport of Phosphatidylserine from Microsomes to the Inner Mitochondrial Membrane in Brain Tissue

Abstract: Phosphatidylserine was labeled by incubating rat brain homogenates with 3-¹⁴C]serine in the presence of Ca²⁺ (base-exchange conditions). Some labeled phosphati-dylethanolamine also forms, in spite of the inhibition of Ca²⁺ on phosphatidylserine decarboxylase. Phosphatidylserine labeling and decarboxylation also occur on incubating a mixture of purified mitochondria and microsomes, suggesting that no soluble factors are necessary for the synthesis and the decarboxylation of phosphatidylserine. Ca²⁺ favors the transfer of phosphatidylserine from microsomes (where it forms) to mitochondria (where it is decarboxylated). The specific radioactivity of the phosphatidylserine transferred to mitochondria is higher than that of microsomal phosphatidylserine. This finding supports the hypothesis that the lipid is compartmentalized in microsomes and that radioactive, newly synthesized phosphatidylserine is much better exported than the bulk of microsomal phospholipid.