An improved enrichment procedure to develop multiple repeat classes of cotton microsatellite markers |
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Authors: | Email author" target="_blank">Siva?P?KumpatlaEmail author Marilyn?K?Manley Erin?C?Horne Manju?Gupta Steven?A?Thompson |
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Institution: | (1) Present address: Department of Trait Genetics and Technologies, Dow AgroSciences LLC, Building 306/C2, 9330 Zionsville Road, 46268 Indianapolis, IN |
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Abstract: | The availability of a large number of molecular markers is a prerequisite, especially in cotton, for identifying a sufficient
number of informative markers for mapping and genetic analysis. Despite the global importance of the cotton crop, few informative
microsatellite markers are available, primarily because of the cost associated with their development. This report describes
an improved and cost-effective strategy for developing microsatellite markers. Genomic DNA was randomly sheared with nitrogen
gas to obtain unbiased representation of the genome, and the fragments containing microsatellites were captured by using biotinylated
oligos and streptavidin-based recovery. Six libraries enriched for 14 microsatellite motifs were constructed and screened.
Nearly 4900 simple sequence repeat (SSR)-containing sequences were identified, leading to the development of more than 1200
markers in a small amount of time. |
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Keywords: | biotinylated oligos cotton enriched libraries microsatellites nebulization polymorphic |
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