OsSPO11-1 is essential for both homologous chromosome pairing and crossover formation in rice |
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Authors: | Hengxiu Yu Mo Wang Ding Tang Kejian Wang Fuli Chen Zhiyun Gong Minghong Gu Zhukuan Cheng |
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Institution: | (1) Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University, Yangzhou, 225009, China;(2) State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China; |
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Abstract: | Spo11 is a homolog of a subunit of archaebacterial topoisomerase, which catalyzes DNA double-strand breaks and initiates homologous
chromosome recombination. In the present study, we silenced the SPO11-1 gene in rice (Oryza sativa) using RNAi. Rice plants with loss-of-function of OsSPO11-1 have no apparent growth defects during vegetative development,
but homologous chromosome pairing and recombination are significantly obstructed. Telomeres can be assembled as bouquet during
the zygotene stage of the OsSPO11-1-deficient plants, just as that in wild type. Although the two axial-associated proteins,
REC8 and PAIR2, are loaded onto the chromosomes, the depletion of PAIR2 from the chromosomes is much later than in wild type.
The central element of the synaptonemal complex (SC), ZEP1, does not load onto the chromosomes normally, implying that SC
formation is disturbed severely. The crossover protein, MER3, isn't efficiently assembled onto chromosomes and the lack of
bivalent suggests that crossovers are also affected in the absence of OsSPO11-1. Thus, OsSPO11-1 is essential for both homologous
chromosomes pairing and crossover formation during meiosis in rice. |
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