Localisation of the MRC OX-2 Glycoprotein on the Surfaces of Neurones |
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Authors: | Michael Webb A Neil Barclay† |
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Institution: | MRC Developmental Neurobiology Unit, London;MRC Cellular Immunology Unit, Sir William Dunn School of Pathology, University of Oxford, Oxford, U.K. |
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Abstract: | The MRC OX-2 monoclonal antibody recognises membrane glycoproteins of Mr 41,000 in rat brain and 47,000 on thymocytes. It also reacts with follicular dendritic cells in lymphoid organs, endothelium, smooth muscle, and B-lymphocytes. Indirect immunoperoxidase staining of cryostat sections showed that OX-2 antigen was present throughout the cerebellum, with staining of both grey and white matter. Blood vessels were also stained. The Purkinje cell layer appeared to be unlabelled. Double-immunofluorescence staining of cerebellar interneurone cultures with MRC OX-2 antibody and tetanus toxin showed that all tetanus-positive cells (neurones) were MRC OX-2-positive. Glial fibrillary acidic protein-positive astrocytes were not labelled by MRC OX-2 antibody. Thus OX-2 antigen is one of the few biochemically characterised components of neuronal membranes and its properties are compared with those of the neuronal membrane glycoprotein Thy-1. |
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Keywords: | Brain Membrane Glycoprotein Neurone MRC OX-2 antigen |
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