Incorporation of single nucleoside triphosphates by isolated cell nuclei

Manganese ions can form insoluble complexes or coprecipitates with nucleoside triphosphates at high pH. We have demonstrated that nucleoside triphosphates found in precipitated complexes exhibit properties similar to those of poliribo-nucleotides in all steps of the Schmidt-Tannhausers procedure: insoluble in perchloric acid, trichloroacetic acid, and ethanol and soluble in 0.2 n NaOH. These observations could be applied to experimentation on RNA synthesis from nucleoside triphosphates.