Plant regeneration from protoplasts of long term haploid suspension cultures of N. plumbaginifolia |
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| Authors: | Donna G. Barfield Susan J. Robinson Robert Shields |
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| Affiliation: | (1) Applied Biosciences, Unilever Research Laboratory, Colworth House, MK44 1LQ Sharnbrook, Bedford, UK |
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| Abstract: | A predominantly haploid cell suspension culture of N.plumbaginifolia has been established. The ploidy has remained stable for nearly four years in culture (and is similar in cells recovered after preservation in liquid nitrogen). Protoplasts isolated from these cells regenerate into plants with a high frequency.Abbreviations MS Murashige and Skoog medium (Murashige and Skoog, 1962) - B5 Gamborg's B5 medium (Gamborg, 1970) - 2,4D 2,4 dichlorophenoxyacetic acid - NAA 1 - Naphthylacetic acid - BAP 6 - Benzylaminopurine, IAA=Indole - 3 - acetic acid - MES (2 (m - morpholino)) ethane sulphonic acid - FDA fluorescein diacetate |
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