The requirement for exopolysaccharide precedes the requirement for flavolan-binding polysaccharide in nodulation of Leucaena leucocephala by Rhizobium loti |
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Authors: | G S Hotter D Barry Scott |
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Institution: | (1) Molecular Genetics Unit, Department of Microbiology and Genetics, Massey University, Palmerston North, New Zealand Tel. +64-6-356-9099; Fax +64-6-350-5637 e-mail: g.s.hotter@massey.ac.nz, NZ |
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Abstract: | Rhizobium loti strain PN4115 (NZP2213 str-1) ineffectively nodulates Leucaena leucocephala, i.e., strain PN4115 induces nodulation (Nod+) and is able to invade these nodules (Inv+), but fails to fix nitrogen (Fix–). Strain PN4115 does not synthesize a flavolan-binding polysaccharide (FBP), which is synthesized by the fully effective
(Nod+Inv+Fix+) R. loti strain PN184 (NZP2037 str-1). The FBP may offer protection from prodelphinidin-rich flavolans synthesized by Lc. leucocephala. In this work, we show that exopolysaccharide (EPS)-negative mutants derived from strain PN4115 have a more severe ineffective
phenotype (Nod+Inv–Fix–) on Lc. leucocephala than strain PN4115. This suggests that EPS from strain PN4115 is functional during invasion of Lc. leucocephala and that the requirement for EPS precedes the requirement for FBP.
Received: 8 October 1996 / Accepted: 11 December 1996 |
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Keywords: | Exopolysaccharide Invasion Tannin Indeterminate nodules Lotus Leucaena Rhizobium |
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