Voltage-activated currents in the CRI-G1 rat insulin-secreting cell-line. |
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Authors: | R Z Kozlowski N C Sturgess C N Hales M L Ashford |
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Institution: | Department of Pharmacology, University of Cambridge, U.K. |
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Abstract: | 1. The whole-cell configuration of the patch-clamp recording technique was used to characterize the electrophysiological properties of CRI-G1 insulin-secreting cells. 2. Current-clamp recordings demonstrated the excitable nature of these cells. 3. Voltage-clamp recordings revealed the presence of an inward Na+ current, an inward Ca2+ current and a delayed outward K+ conductance. 4. The electrophysiological properties of CRI-G1 closely resemble those of pancreatic beta-cells, thereby rendering this cell-line as a useful alternative to freshly isolated cells for the study of pancreatic beta-cell electrophysiology and pharmacology. |
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