| 千层塔内生真菌SHB发酵产石杉碱甲条件的研究 |
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| 引用本文： | 周树良,杨帆,兰时乐,徐宁,洪亚辉.千层塔内生真菌SHB发酵产石杉碱甲条件的研究[J].微生物学杂志,2009,29(3):32-36. |
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| 作者姓名： | 周树良 杨帆 兰时乐 徐宁 洪亚辉 |
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| 作者单位： | 湖南农业大学,生物科学技术学院,湖南,长沙,410128 |
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| 摘 要： | 为提高千层塔产黄青霉属内生真菌SHB液体发酵产石杉碱甲的产量，通过单因子和正交试验研究了真菌SHB液体发酵的条件。结果表明，千层塔内生真菌SHB液体发酵产石杉碱甲的适宜条件：温度为28℃，起始pH为6．4，接种量为12％，种龄为48h，转速为160r／min，发酵时间为8d。在此条件下，石杉碱甲的产量可达4．761μg／mL。
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| 关 键 词： | 千层塔 产黄青霉属 发酵 石杉碱甲 |
Huperzine A Producing Conditions from Endophytic Fungus in SHB Huperzia Serrata |
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| ZHOU Shu-liang,YANG Fan,LAN Shi-le,XU Ning,HONG Ya-hui.Huperzine A Producing Conditions from Endophytic Fungus in SHB Huperzia Serrata[J].Journal of Microbiology,2009,29(3):32-36. |
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| Authors: | ZHOU Shu-liang YANG Fan LAN Shi-le XU Ning HONG Ya-hui |
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| Institution: | (Coll. of Biosci. ＆ Biotechnol. , Htman Agric. Univ., Changsha 410128) |
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| Abstract: | In order to increase the output of Huperzine A by endophytic fungus SHB (Penicillium chrysogenum) from Huperzia serrata (Lycopodium serratum), the liquid fermentation conditions was studied through single factors and orthogonal experiment. The results showed that the most suitable liquid fermentation conditions of SHB to produce Huperzine A were 28 ℃, starting pH at 6.4, inoculation volume at 12%, strain seed instar at 48 h, 160 rpm to ferment for 8 d. Under these conditions the yield of Huperzine A could reach 7.210 μg/mL. |
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| Keywords: | Huperzia serrata (Lycopodium serratum) Penicillium chrysogenum fermentation Huperzine A |
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