Definition of the T-lymphocyte inducer of suppression in primates using a monoclonal antibody |
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| Authors: | N L Letvin C Morimoto W R Aldrich S F Schlossman |
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| Affiliation: | 1. Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115, U.S.A.;2. New England Regional Primate Research Center, Southborough, Massachusetts 01772, U.S.A.;1. Department of Veterinary & Animal Sciences, University of Massachusetts, Amherst, MA, USA;2. The Pirbright Institute, Pirbright, Surrey, UK;3. Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria;4. Division of Hematology, University of Utah School of Medicine, Salt Lake City, UT, USA;1. National Animal Disease Center, USDA/ARS, Ames, IA, USA;2. US Navy Marine Mammal Program, Naval Information Warfare Center Pacific, San Diego, CA, USA;3. Kingfisher Biotech, Inc., St. Paul, MN, USA;4. Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA, USA |
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| Abstract: | Since some of the conserved antigens between man and phylogenetically lower primate species may be more immunodominant on lymphocytes of the lower primate species, we reasoned that immunization of mice with lymphocytes from lower primates might prove a useful strategy for developing monoclonal antibodies which recognize functionally important structures on both human and nonhuman primate lymphocytes. In employing this approach for the development of monoclonal antibodies, we have developed the antibody anti-2H4 which recognizes a structure on both T on non-T mononuclear cells of a wide array of primate species. 2H4+ rhesus monkey T lymphocytes exhibited a greater proliferative response to lectin and alloantigenic stimulation than 2H4- cells, suggesting that anti-2H4 might separate primate T lymphocytes into functionally distinct cell populations. In fact, helper activity for antibody production by rhesus monkey B lymphocytes in response to pokeweed mitogen (PWM) resided in the 2H4- T-cell population. Furthermore, the 2H4+ T-lymphocyte population activated the suppressor function of T8+ rhesus monkey cells. The fact that the surface antigen which defines this T-cell subset is widely conserved in nonhuman primates suggests that anti-2H4 recognizes a functionally important structure. |
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