Determination of growth hormone-releasing hexapeptide by reversed-phase high-performance liquid chromatography with electrochemical detection |
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| Authors: | Sung Jin Choi Hee-Yong Lee Sang Beam Kim Jeong-Han Kim Seung Seok Lee Sun Dong Yoo Kang Choon Lee Hye Suk Lee |
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| Affiliation: | 1. Bioanalysis Laboratory, College of Pharmacy, Wonkwang University, Iksan 570-549, South Korea;2. School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, South Korea;3. College of Pharmacy, SungKyunKwan University, Suwon 440-746, South Korea;1. The Key Laboratory of Applied Chemistry of Chongqing Municipality, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China;2. Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Ave., Kowloon, Hong Kong, China;3. Department of Science and Environmental Studies, The Education University of Hong Kong, 10 Lo Ping Road, Tai Po, Hong Kong, China;1. Technical University of Crete, School of Environmental Engineering, 73100 Chania, Greece;2. Technological Educational Institute of Crete, Department of Environmental and Natural Resources Engineering, 73133 Chania, Greece;3. University of Crete, Department of Chemistry, 71003 Heraklion, Greece;4. Technological Educational Institute of Athens, Department of Conservation of Antiquities and Works of Art, 12210 Egaleo, Greece;1. Department of Chemistry, Dwaraka Doss Goverdhan Doss Vaishnav College (Autonomous) (Affiliated to University of Madras), 833, Gokul Bagh, E.V.R.Periyar Road, Arumbakkam, Chennai, 600106, Tamil Nadu, India;2. Department of Biotechnology, Dwaraka Doss Goverdhan Doss Vaishnav College (Autonomous) (Affiliated to University of Madras), 833, Gokul Bagh, E.V.R. Periyar Road, Arumbakkam, Chennai, 600106, Tamil Nadu, India;1. Department of Vascular Surgery, Turku University and Turku University Hospital, Turku, Finland;2. Medicity Research Laboratory, Department of Microbiology and Immunology, University of Turku, Turku, Finland |
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| Abstract: | A novel HPLC method with electrochemical detection is described for the determination of a growth-hormone-releasing hexapeptide (GHRP-6). HPLC conditions, such as the column, mobile phase, and oxidation potential, were optimized for sensitivity and selectivity of analysis. GHRP-6 was separated on a reversed-phase CN column with 37% acetonitrile in 100 mM phosphate buffer (pH 7.0) as the mobile phase. The optimum electrochemical oxidation signal was obtained at 0.85 V vs. Ag/AgCl in a glassy carbon working electrode due to two electroactive tryptophans and a histidine residue. Solid-phase extraction using octadecyl cartridges was optimized for sample cleanup of GHRP-6 from serum samples and the method was successfully applied over the concentration range of 5 to 100 ng/ml of analyte. |
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| Keywords: | Growth-hormone-releasing peptide |
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