Effect of various surfactants (cationic,anionic and non-ionic) on the growth of <Emphasis Type="Italic">Aspergillus parasiticus</Emphasis> (NRRL 2999) in relation to aflatoxin production |
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Authors: | Kosuri Tanuja K Hemalatha Rupula Karuna B Sashidhar Rao |
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Institution: | (1) Department of Biochemistry, University College of Science, Osmania University, Hyderabad, 500 007, Andhra Pradesh, India; |
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Abstract: | The effect of surfactants (two cationic, one anionic and three non-ionic) at 0.001, 0.01, 0.1 and 1.0 % concentrations on
aflatoxin production, ergosterol content and sugar consumption by Aspergillus parasiticus (NRRL 2999) in YES liquid culture medium is reported. At 0.01% concentration, the cationic surfactants, cetyl dimethyl ammonium
bromide (CDAB) and dodecyl trimethyl ammonium bromide (DTAB), and the anionic surfactant, sodium dodecyl sulfate (SDS), completely
inhibited spore germination, while DTAB also inhibited the production of ergosterol and toxin (p < 0.05). At a concentration of 0.001%, CDAB was found to enhance toxin production, while SDS was found to inhibit it when
compared with other surfactants. Non-ionic surfactants, polyoxyethylene sorbitan monolaurate (Tween-20), polyoxyethylene lauryl
ether (Brij-35) and ethoxylated p-tert-octylphenol (Triton X-100) delayed the spore germination up to day 5 at all concentrations and inhibited toxin and ergosterol
production at 0.001% concentration. The affect was found to be dose-dependent from 0.001% to 1%, for Triton X-100 only. Positive
correlation between ergosterol content and toxin production in the presence of different surfactants at various time periods
(3, 5, 7, 9 and 12 days) was found. Tween-20 was most effective in inhibiting toxin production on day 7, when aflatoxin production
was found to be maximal in control group. Sugar consumption was directly proportional to the ergosterol content, showing a
significant correlation with aflatoxin production. |
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Keywords: | |
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