Deoxynivalenol loads in matched pair wheat samples in Belgium: comparison of ELISA VERATOX kit against liquid chromatography |
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Authors: | Emmanuel K. Tangni Jean-Claude Motte Alfons Callebaut Anne Chandelier Marnix De Schrijver Luc Pussemier |
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Affiliation: | (1) Chemical Safety of the Food Chain, Unit of Toxins and Natural components, Veterinary and Agrochemical Research Centre (CODA-CERVA), Leuvensesteenweg 17, 3080 Tervuren, Belgium;(2) Department Biological Control and Plant Genetic Resources, Walloon Agricultural Research Centre (CRA-W), Rue de Liroux 4, 5030 Gembloux, Belgium;(3) Food and Feed Quality (FFQ) Laboratorium, E. Meeusstraat 6, 2170 Merksem, Belgium |
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Abstract: | A comparison of matched pairs deoxynivalenol (DON) loads in wheat samples via VERATOX for DON 5/5 performed by two laboratories against two liquid chromatographic methods (LC-MS/MS and HPLC-UV) used by two other laboratories was carried out using biometrical and sum of ranking differences (SRD) procedures. The Lin’s Concordance correlation coefficients, the average discrepancies, the limits of agreement and the SRD between ELISA and reference values showed good overall agreement between VERATOX for DON 5/5 and reference methods for the two datasets. The VERATOX kits are valuable for quantitative screening and even for an initial exposure assessment in situations when there are practical or economical reasons not to use sophisticated methods such as HPLC or GC methods (with or without MS). However, networking of laboratories using this rapid method and laboratories with reference analytical methods should be encouraged. |
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