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Characterization of an F18+ enterotoxigenic Escherichia coli strain from post weaning diarrhoea of swine, and of its conjugative virulence plasmid pTC
Authors:Olasz Ferenc  Fekete Péter Zsolt  Blum-Oehler Gabriele  Boldogkoi Zsolt  Nagy Béla
Institution:1. Institute of Environmental Biosafety, Agricultural Biotechnology Center, Szent-Györgyi Albert st. 4, H-2101 Gödöll?, Hungary;2. Veterinary Medical Research Institute of Hungarian Academy of Sciences H-1143 Budapest Hungária krt. 21, Hungary;3. Institut für Molekulare Infektionsbiologie, D-97070 Würzburg, Röntgenring 11, Germany;1. Platform for Artificial Insemination and Embryo Transfer, Vetmeduni Vienna, Vienna, Austria;2. Dept of Obstetrics and Gynaecology, Adnan Menderes University, Faculty of Veterinary Medicine, Ayd?n, Turkey;3. Dept of Histology and Embryology, Harran University, Faculty of Veterinary Medicine, Sanliurfa, Turkey;4. Dept of Pathobiology, Institute of Anatomy, Histology and Embryology, University of Veterinary Medicine, Vienna, Austria;5. VetBiobank, Vetcore, University of Veterinary Medicine, Vienna, Austria;6. Department of Obstetrics and Gynecology, Veterinary Faculty, Near East University, Mersin-10, Turkey;1. Nutreco Research and Development, P.O. Box 220, 5830 AE, Boxmeer, the Netherlands;2. Wageningen UR Livestock Research, P.O. Box 65, 8200 AB, Lelystad, the Netherlands;3. Animal Nutrition Group, Wageningen University, 6709 PG Wageningen, the Netherlands;4. Adaptation Physiology Group, Wageningen University, 6700 AH Wageningen, the Netherlands;1. Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China;2. Beijing Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Beijing 100193, China;3. Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130000, China;1. Food Animal Health Research Program, Ohio Agricultural Research and Development Center, College of Food, Agricultural and Environmental Sciences, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, USA;2. Division of Virology and Epidemiology, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan;3. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China;1. Sherbrooke Research and Development Centre, Agriculture and Agri-Food Canada, Sherbrooke, Quebec J1M 0C8, Canada;2. Biology Department, Faculty of Sciences, Université de Sherbrooke, 2500 boul. de l’Université, Sherbrooke, Quebec J1K 2R1, Canada;3. Quebec’s Animal Pharmacology Research Group (GREPAQ), Department of Veterinary Biomedicine, Faculty of Veterinary Medicine, Université de Montréal, 3200 Sicotte, Saint-Hyacinthe, Quebec J2S 2M2, Canada
Abstract:The enterotoxigenic Escherichia coli (ETEC) strain Ec2173, causing post weaning diarrhoea in swine, harbours six plasmids ranging from 13 to 200 kb in size. The heat stable toxin genes sta, stb and a tetracycline resistance gene were located on a self conjugative 120-kb plasmid, called pTC. In the cloned ColE1 type origin of replication of pTC a deletion was detected compared to other ColE1 replicons affecting the replication modulator gene rom. Epidemiological studies on ETEC isolates showed that pTC-like plasmids are widely distributed among porcine ETEC strains; thus representing an example of co-evolution of antibacterial resistance and virulence in pathogenic E. coli.
Keywords:ETEC  Heat stable toxin gene  Virulence plasmid  Origin of replication  Maintenance of plasmids  Tetracycline
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