Protein Phosphorylation Is Induced in Tobacco Cells by the Elicitor Cryptogein |
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Authors: | Viard M P Martin F Pugin A Ricci P Blein J P |
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Institution: | Institut National de la Recherche Agronomique, Universite de Bourgogne, Laboratoire de Phytopharmacie Phytobiologie Cellulaire, BV 1540 21034 Dijon Cedex, France (M.-P.V., F.M., A.P.J.-P.B.). |
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Abstract: | Changes in plasmalemma ion fluxes were observed when tobacco (Nicotiana tabacum) cells were treated with cryptogein, a proteinaceous elicitor from Phytophthora cryptogea. A strong alkalization of the culture medium, accompanied by a leakage of potassium, was induced within a few minutes of treatment. These effects reached a maximum after 30 to 40 min and lasted for several hours. This treatment also resulted in a rapid, but transient, production of activated oxygen species. All these physiological responses were fully sensitive to staurosporine, a known protein kinase inhibitor. Furthermore, a study of protein phosphorylation showed that cryptogein induced a staurosporine-sensitive phosphorylation of several polypeptides. These data suggest that phosphorylated proteins may be essential for the transduction of elicitor signals. |
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