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Enhanced shoot and cormlet proliferation in liquid cultured gladiolus buds by growth retardants
Authors:Meira Ziv
Affiliation:(1) Department of Agricultural Botany, The Hebrew University, 76100 Rehovot, Israel;(2) The Warburg Center for Biotechnology in Agriculture, The Hebrew University, 76100 Rehovot, Israel
Abstract:Gladiolus bud explants propagated in agitated liquid medium, in the presence of the growth retardants daminozide, ancymidol, paclobutrazol or Majic, proliferated into massive bud aggregates without leaves. The buds developed into protocorms and after subculture to agar-solidified medium formed cormlets 8–10 mm in diameter. The corms were not dormant and, after transplanting to pots in the greenhouse, developed into plantlets, 5–6 months after explant isolation.
Keywords:liquid cultures  gladiolus  bud proliferation  growth retardants  cormlets
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