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| Bt水稻中crylAb基因的遗传分析 | |
| 引用本文： | 王忠华,吴刚,崔海瑞,夏英武,舒庆尧. Bt水稻中crylAb基因的遗传分析[J]. 遗传学报, 2001, 28(9): 846-851 |
| 作者姓名： | 王忠华 吴刚 崔海瑞 夏英武 舒庆尧 |
| 作者单位： | 1. 浙江大学原子核农业科学研究所 2. 北京大学 3. 加拿大渥太华大学生物化学系 |
| 基金项目： | 浙江省科委重大项目(991102004-002)和美国洛氏 |
| 摘 要： | 用PCR、GUS染色和Western点杂交技术检测了Bt水稻杂交后代群体，在394株GUS阳性株中，有392株表达Bt蛋白，协同表达株率达99.49%。由此表明，在杂交后代中报告基因gus和目的基因crylAb紧密连锁遗传与表达。GUS组织染色和Southern杂交检测表明Bt水稻中的crylAb基因呈单位点显性基因遗传，且在有性世代中能稳定传递，还发现，在BC1、BC1F2和粳粳交F2群体中crylAb基因呈单位点显性基因遗传，而在籼粳交F2群体中偏离3：1分离。 |
| 关 键 词： | Bt水稻 crylAb基因 遗传特点 育种 |
| 文章编号： | 0379-4172(2001)-09-0846-06 |
| 修稿时间： | 2001-01-08 |
Genetic Analysis of cry1Ab Gene of Bt Rice |
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| WANG Zhong-Hua,WU Gang,CUI Hai-Rui,Illimar Altosaar,XIA Ying-Wu,SHU Qing-Yao. Genetic Analysis of cry1Ab Gene of Bt Rice[J]. Journal of Genetics and Genomics, 2001, 28(9): 846-851 | |
| Authors: | WANG Zhong-Hua WU Gang CUI Hai-Rui Illimar Altosaar XIA Ying-Wu SHU Qing-Yao |
| Abstract: | Improved histochemical staining for GUS activity, PCR and Western blotting were used to analyse the progeny population of Bt rice crossed with conventional rice varieties. A total of 392 plants expressing Bt toxin protein were found in 394 GUS postive plants. The result demonstrated that crylAb gene closely inherited and expressed with reporter gene gus. GUS assays indicated that crylAb gene of Bt rice inherits as a single dorminant gene. Southern hybridization confirmed crylAb gene can inherit stabily in the progenies of Bt rice. Mendelian segregation of reporter gene gus was also observed in F2, BC1 and BC1F2 progenies, which indicated that crylAb gene inherits as a single dorminant gene in the progenies of Bt rice crossed with conventional rice varieties. |
| Keywords: | Bt rice cry1Ab gene genetic trait breeding |
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