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| 粘附分子CD146是影响滋养层细胞侵入行为的关键分子 | |
| 引用本文： | 刘琴,赵心刚,张莹,沈毅,刘以训,阎锡蕴.粘附分子CD146是影响滋养层细胞侵入行为的关键分子[J].生物化学与生物物理进展,2004,31(4):309-312. |
| 作者姓名： | 刘琴 赵心刚 张莹 沈毅 刘以训 阎锡蕴 |
| 作者单位： | 1. 中国科学院生物物理研究所,生物大分子国家重点实验室,北京,100101;中国科学院微生物研究所,微生物资源前期开发国家重点实验室,北京,100080 2. 中国科学院生物物理研究所,生物大分子国家重点实验室,北京,100101 3. 中国科学院动物研究所,计划生育生殖生物学国家重点实验室,北京,100080 |
| 基金项目： | 国家自然科学基金资助项目（30070387）和国家高技术“863计划”资助项目（2001AA215321）. |
| 摘 要： | 前期研究观察到一种现象, 在正常妊娠的胎盘中细胞粘附分子CD146选择性地表达在侵入性滋养层细胞中, 而在滋养层细胞侵入不足的先兆子痫病人的胎盘中CD146表达降低或缺失.本文进一步研究了CD146分子影响滋养层细胞侵入行为的作用机理.免疫荧光实验显示CD146分子选择性地表达在具有侵袭能力的中间滋养层细胞,而在非侵入性的细胞滋养层细胞和合体滋养层细胞中不表达.细胞功能实验表明,影响滋养层细胞侵入性的两个关键要素,即细胞迁移和基质金属蛋白酶的分泌,都受到CD146特异抗体的显著抑制.这些研究结果提示，粘附分子CD146是影响细胞侵入行为的关键分子.这为深入研究胚胎植入和肿瘤浸润的分子调控机理提供了一个关键的分子模型. |
| 关 键 词： | 细胞粘附分子CD146 滋养层细胞侵入 基质金属蛋白酶 |
| 收稿时间： | 3/9/2004 12:00:00 AM |
| 修稿时间： | 2004年3月9日 |
Melanoma Cell Adhesion Molecule (MCAM/CD146) Is a Critical Molecule in Trophoblast Invasion |
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| LIU Qin,ZHAO Xin-Gang,ZHANG Ying,SHEN Yi,LIU Yi-Xun and YAN Xi-Yun.Melanoma Cell Adhesion Molecule (MCAM/CD146) Is a Critical Molecule in Trophoblast Invasion[J].Progress In Biochemistry and Biophysics,2004,31(4):309-312. | |
| Authors: | LIU Qin ZHAO Xin-Gang ZHANG Ying SHEN Yi LIU Yi-Xun and YAN Xi-Yun |
| Institution: | National Laboratory of Biomacromolecules, Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China;State Key Laboratory of Microbial Resources, Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080, China;National Laboratory of Biomacromolecules, Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China;National Laboratory of Biomacromolecules, Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China;National Laboratory of Biomacromolecules, Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China;State Key Laboratory of Reproduction Biology, Institute of Zoology, The Chinese Academy of Sciences, Beijing 100080, China;National Laboratory of Biomacromolecules, Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China |
| Abstract: | Previous study has shown that melanoma cell adhesion molecule (MCAM/CD146) is highly expressed by invasive trophoblasts in normal pregnancy, but it is absent in pregnancy disorder pre-eclampsia in which the invasion ability of trophoblast is seriously impaired. The mechanism that CD146 affects the trophoblast invasion was investigated. Immunofluorescence staining revealed that CD146 was selectively expressed on invasive intermediate trophoblasts, but was not expressed on non-invasive cytotrophoblasts and syncytiotrophoblasts. Functional studies showed that the anti-CD146 antibody significantly inhibited the migration of trophoblasts and the activity of matrix metalloproteinases secreted by trophoblastic cells, which are two critical factors for trophoblast invasion. The results demonstrate that adhesion molecule CD146 plays a critical role in trophoblast invasion, which will provide a valuable molecular model for the study of trophoblast and tumor cell invasion. |
| Keywords: | CD146 trophoblast invasion matrix metalloproteinase |
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