Outer membrane protein profiles of clonally related Klebsiella pneumoniae isolates that differ in cefoxitin resistance |
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Authors: | Skopková-Zarnayová Martina Siebor Eliane Rovná Daniela Bujdáková Helena Neuwirth Catherine |
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Affiliation: | Department of Microbiology and Virology, Comenius University, Mlynska dolina B-2, 845 15 Bratislava, Slovakia. martina.skopkova@savba.sk |
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Abstract: | Eleven genotypically related Klebsiella pneumoniae isolates were obtained from 11 patients. All isolates were resistant to third-generation cephalosporins due to the production of SHV-2a extended-spectrum beta-lactamase. Comparison of the outer membrane protein profiles revealed one isolate that lacked porins. This porin-deficient isolate was also resistant to cefoxitin (MIC 128 microg ml(-1)) and moxalactam (MIC 64 microg ml(-1)) and had elevated MIC of meropenem (2 microg ml(-1)) when compared to porin-expressing isolates (2-8, 4 and <0.06-0.125 microg ml(-1), respectively). Higher MICs, associated with loss of porins in outer membrane, were also observed for cefotaxime (4-8-fold), cefepime (>2-16-fold), ciprofloxacin (4-16-fold), imipenem and aztreonam (2-16-fold), but there was no significant difference among MICs of ceftazidime. The porin-deficient mutant was probably selected in vivo during ofloxacin therapy. |
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Keywords: | Klebsiella pneumoniae Bacterial porin Multiresistance β-Lactam antibiotic ESBL Hospital-acquired infection |
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