Theoretical analysis of dynamic force spectroscopy experiments on ligand-receptor complexes |
| |
Authors: | Raible M Evstigneev M Reimann P Bartels F W Ros R |
| |
Institution: | Theoretische Physik, Universit?t Bielefeld, Universit?tsstrasse 25, 33615 Bielefeld, Germany. |
| |
Abstract: | The forced rupture of single chemical bonds in biomolecular compounds (e.g. ligand-receptor systems) as observed in dynamic force spectroscopy experiments is addressed. Under the assumption that the probability of bond rupture depends only on the instantaneously acting force, a data collapse onto a single master curve is predicted. For rupture data obtained experimentally by dynamic AFM force spectroscopy of a ligand-receptor bond between a DNA and a regulatory protein we do not find such a collapse. We conclude that the above mentioned, generally accepted assumption is not satisfied and we discuss possible explanations. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|