Purification and characterization of heparinase that degrades both heparin and heparan sulfate from Bacillus circulans |
| |
Authors: | Yoshida Eiichi Sakai Kazuya Tokuyama Shinji Miyazono Hirofumi Maruyama Hiroshi Morikawa Kiyoshi Yoshida Keiichi Tahara Yasutaka |
| |
Affiliation: | Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Japan. |
| |
Abstract: | A heparinase that degrades both heparin and heparan sulfate (HS) was purified to homogeneity from the cell-free extract of Bacillus circulans HpT298. The purified enzyme had a single band on SDS-polyacrylamide gel electrophoresis with an estimated molecular mass of 111,000. The enzyme showed optimal activity at pH 7.5 and 45 degrees C, and its activity was stimulated in the presence of 5 mM CaCl2, BaCl2, or MgCl2. Analysis of substrate specificity and degraded disaccharides demonstrated that the enzyme acts on both heparin and HS, similar to heparinase II from Flavobacterium heparinum. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|