Cloning, expression, and crystallization of a hyperthermophilic protein that is homologous to the eukaryotic translation initiation factor, eIF5A. |
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| Authors: | K K Kim H Yokota R Kim and S H Kim |
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| Institution: | Structural Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA. |
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| Abstract: | A gene coding for a protein homologous to a translation initiation factor of eukaryotes, eIF5A, was cloned from Methanococcus jannaschii, a hyperthermophile with an optimum growth temperature of 85 degrees C. The protein was overexpressed, purified and crystallized. The crystals were obtained by vapor diffusion method with 8% PEG 4000 as precipitant and belong to space group P4(1)22 with unit cell dimensions a = b = 45.52 A and c = 155.59 A. These crystals diffract to at least 2.2 A resolution. |
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| Keywords: | hyperthermophile Methanococcus jannaschii translation initiation factor X-ray crystallography |
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