Metabolism of pyruvate by rat adipose tissue in vitro |
| |
| Authors: | L W White H R Williams B R Landau |
| |
| Affiliation: | 1. MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Collaborative Innovation Center of Chemistry for Energy Materials, Key Laboratory for Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China;2. Xiamen Entry-Exit Inspection and Quarantine Bureau, Xiamen 361026, China;1. Central Laboratories, King Faisal University, Al-Ahsa 31982, Saudi Arabia;2. School of Biochemistry and Biotechnology, University of the Punjab, Quaid-e-Azam campus, P.O. Box 54590, Lahore, Pakistan;3. National Institute for Biotechnology and Genetic Engineering (NIBGE), 38000 Faisalabad, Pakistan;4. Department of Biotechnology, Faculty of Sciences, University of Sialkot, Sialkot, Pakistan |
| |
| Abstract: | Pyruvate-1-14C, pyruvate-2-14C and pyruvate-3-14C, and unlabeled pyruvate in the presence of 14CO2, were incubated in a bicarbonate buffer with rat epididymal adipose tissue. Incorporation of 14C into CO2, glycerol and fatty acids of triglycerides, lactate and glycogen were measured and the glycerol and lactate were degraded to yield the relative activities of 14C in each of their carbons. The results are in accord with metabolism via the Embden-Meyerhof Pathway and Krebs Cycle. The dicarboxylic acid shuttle, involving conversion of pyruvate to phosphoenolpyruvate via CO2 fixation with oxaloacetate as an intermediate, and equilibration with symmetrical dicarboxylic acids of the Krebs Cycle, occurs in adipose tissue and plays a major role in the distribution of the carbons of pyruvate. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
