Recovery of transferrin receptors on hepatocytes membrane after collagenase perfusion |
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| Authors: | T Kishimoto M Tavassoli |
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| Affiliation: | 1. College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China;2. Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, PR China;3. ShenZhen Water(Group) Co., LTD, ShenZhen 518000, PR China;1. Department of Botany, Bangalore University, Bengaluru, 560 056, India;2. Molecular Diagnostics and Nanobiotechnology Laboratories, Department of Microbiology and Biotechnology, Bangalore University, Bangalore, 560056, India;3. Centre for Advanced Materials Research Lab, Department of Physics, BMS Institute of Technology and Management, Bengaluru, 560 064, India;4. Research Center, Department of Science, East West Institute of Technology, Bangalore, 560091, India;1. School of Mechanical Engineering, University of Science and Technology Beijing, PR China;2. Beijing Key Laboratory of Lightweight Metal Forming, Beijing, PR China;3. Department of Mechanical Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong |
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| Abstract: | Hepatocyte cell suspensions obtained by collagenase perfusion method did not have transferrin (TF) receptors. However, after incubation at 37 degrees, they appeared to gain TF receptors, the number of which was the function of incubation time at 37 degrees. It is suggested that hepatocyte TF receptors are collagenase-sensitive. This study can explain previous observations that hepatocyte isolated with collagenase treatment of the tissue do not bind TF at 4 degrees but take up TF at 37 degrees. |
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