Genetic Linkage Maps of <Emphasis Type="Italic">Betula platyphylla</Emphasis> Suk Based on ISSR and AFLP Markers |
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Authors: | Zhigang Wei Kaixuan Zhang Chuanping Yang Guifeng Liu Guanjun Liu Lian Lian Hanguo Zhang |
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Institution: | (1) Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Northeast Forestry University, Ministry of Education, Harbin, Hei Longjiang Province, China |
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Abstract: | Two separate genetic linkage maps for Chinese silver birch based on inter-simple sequence repeat (ISSR) and amplified fragment-length
polymorphism (AFLP) were constructed by a pseudo-testcross mapping strategy. Eighty F1 progenies were obtained from the cross between two parental trees with desirable traits (the paternal one selected from ‘Qinghai’
and the maternal one from ‘Wangqing’). A total of 46 ISSR primers and 31 AFLP primers were employed to generate 102 ISSR and
355 AFLP polymorphic markers in the F1 progenies. About 5.7% of all the markers displayed high segregation distortion with a P value below 0.01 and such markers were not used for map constructions. The paternal map consisted of 137 loci, spread over
13 groups and spanned 694.2 cM at an average distance of 5.1 cM between the markers, while in the maternal map, 147 loci were
distributed in 14 groups covering a map distance about 949.62 cM at an average distance of 6.5 cM. These initial maps can
serve as the basis for developing a more detailed genetic map. |
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