植物病原棒形细菌的RAPD分析

采用RAPD分析技术对萎蔫短小杆菌落葵致病变种（Curtobacterium flaccumfaciens pv. basellae pv. Nov.）和萎蔫短小杆菌糖甜菜致病变种（Curtobacterium flaccumfaciens pv. beticola pv. Nov.）及植物病原棒形细菌4个属的15个菌株进行分类研究：从50条随机引物中筛选出20条，共产生225条带，多态性条带占804%。遗传相似矩阵及UPGMA聚类分析，表明这两个新致病变种与萎蔫短小杆菌属亲缘关系近，最小相似系数为0.6511；与其他属细菌亲缘关系较远；结合前人研究结果对植物病原棒形细菌新近提出的分类地位的改变进行了探讨。

RAPD analysis of plant pathogenic coryneform bacteria

RAPD analysis was used for the taxonomy of plant pathogenic coryneform bacteria, especially for the classification of two new pathogens (Curtobacterium flaccumfaciens pv. basellae pv. nov. and Curtobacterium flaccumfaciens pv. beticola pv. nov.). 20 random primers were screened from 50 ones to detect polymorphism among the total strains used. 80.4% were polymorphic bands among the 225 ones produced. The results of pairwise similarity and UPGMA cluster analysis suggest that the two new pathovars of sugar beet (Beta vulgaris var. saccharifera) and malabar spinach (Basella rubra) are genetically close related with Curtobacterium flacumfaciens, and the minimal similarity coefficient is 0.6511. According to the RAPD analysis and previous research, some newly made taxonomic changes of the plant pathogenic coryneform bacteria are discussed.