in Vitro Activity of Antimycotic Agents Determined by e-Test Method Against Vaginal Shape Candida Species |
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Authors: | Candido Regina Celia Toloi Maria Regina Torqueti Franceschini Silvio Antonio Garcia Flaviane Ramos Minto Elaine Cristina Manini |
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Institution: | 1.Departamento de Análises Clínicas, Toxicológicas e Bromatológicas da Faculdade de Ciências Farmacêuticas de Ribeir?o Preto, Universidade de S?o Paulo, SP, Brasil ;2.Sistema Integrado de Saúde da Universidade de S?o Paulo (SISUSP – Ribeir?o Preto), Brasil ; |
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Abstract: | Vaginal candidiasis continues to be a common cause of vaginal discharge, pruritus and other local complaints in women worldwide.
Although numerous antimycotic agents are available for the treatment of yeast vaginitis there is little comparative data on
the in vitro activity of these drugs. The objectives of this study were to isolate and identify the Candida species in the
vagina and anus of patients treated in a gynaecology clinic, as well as determine the susceptibility to azolic compounds measured
by the E-test method. Vaginal and rectal swabs were collected from 80 adult non-pregnant patients, seen at a gynaecological
clinic, aged 18–59 years, with sexual activity, with and without vaginitis. The swabs were processed by methods routinely
used for the detection of pathogenic yeasts. The susceptibility of the isolates to fluconazole, ketoconazole and itraconazole,
was measured by the agar diffusion method (E-test), using RPMI 1640 medium with 2% glucose and phosphate buffer. Candida species
(33) strains were isolated from 17 patients at similar proportions from both anatomical sites, and 12 patients harboured 24
strains of C. albicans in the vaginal and rectal tracts. Twenty one percent of the strains of C. albicans were resistant to
ketoconazole, 54% were resistant to itraconazole and 0% were resistant to fluconazole. The sensitivity of strains isolated
from the two sites were similar, indicating that these are strains of the same phenotype.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | C albicans C krusei C parapsilosis C tropicalis E-test yeasts |
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