Ketamine-induced cardiac depression is associated with increase in [Mg2+]i and activation of p38 MAP kinase and ERK 1/2 in guinea pig |
| |
Authors: | Kim Shang-Jin Kang Hyung-Sub Lee Mun-Young Lee Sei-Jin Seol Jea-Won Park Sang-Yeol Kim In-Shik Kim Nam-Soo Kim Sung-Zoo Kwak Yong-Geun Kim Jin-Shang |
| |
Affiliation: | College of Veterinary Medicine, Chonbuk National University, Jeonju 561-756, Republic of Korea. |
| |
Abstract: | This study investigated the signaling pathways responsible for ketamine-induced cardiac depression in guinea pigs. The left ventricular development pressure (LVDP), velocity of the change in pressure (dP/dt), and heart rate (HR) accompanied with the total magnesium efflux ([Mg]e) were measured simultaneously in perfused hearts. The level of activation of the extracellular signal-regulated kinases 1/2 (ERK 1/2) and p38 mitogen-activated protein (MAP) kinase. The intracellular ionized magnesium concentration ([Mg2+]i) was measured using Mag-fura 2 AM in a single cardiomyocyte. Ketamine produced reversible decreases in the LVDP, dP/dt, and HR accompanied by increases in the [Mg]e. Ketamine also produced significant activation of p38 MAP kinase and ERK 1/2, and produced a dose-dependent increase in the [Mg2+]i, which was inhibited SB203580 and PD98059. These results suggest that ketamine-induced cardiac depression can be partly responsible for the increase in [Mg2+]i and [Mg]e, accompanied by the activation of p38 MAP kinase and ERK 1/2 in guinea pigs. |
| |
Keywords: | Ketamine Cardiac depression Intracellular magnesium ion p38 MAP kinase ERK 1/2 |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|