向日葵下胚轴原生质体高频率愈伤组织形成与根发生

向日葵籽苗下胚轴原生质体，培养在含有ＢＡ０．５ｍｇ／Ｌ，２，４－Ｄ０．５ｍｇ／Ｌ，ＮＡＡ０．１ｍｇ／Ｌ和葡萄糖０．５５ｍｇ／Ｌ的改良Ｋａｏ培养基中，２４～２８ｈ后，原生质体开始分裂。包埋在琼脂糖０．６％中的原生质体，培养５ｄ后，分裂频率达９５％以上。生长旺盛的小愈伤组织转移到含有２ｉｐ０．１ｍｇ／Ｌ，ＩＡＡ０．０１ｍｇ／Ｌ，腺嘌呤４０ｍｇ／Ｌ和ＧＡ３０．０１ｍｇ／Ｌ的Ｔｈｏｍｐｓｏｎ液体培养基上１３ｄ后，原生质体诱导的少数愈伤组织发生根分化。

High Frequency Callus Formation and Limited Rhizogenesis withHypocotyl Protoplasts of Helianthus annuus L.

Protoplasts isolated fromhypocotyls of Helianthus annuus seedlings grown in the dark were cultured on modified mediumafter Kao (1982) containing BA 0. 5 mg/L,2, 4-D 0. 5 mg/L, NAA 0.1 mg/L and glucose 0. 55 mol/L as the sole source of caralhydrates. Protoplasts started to divide after 24-28 h of culture and after five days 95% ofprotoplasts immobilized in 0. 6% agarose haddivided at least once. Rapidly growing microcalli were transferred to liquid medium afterThomPSon et al. (1986) containing 2-ip 0. 1mg/L, IAA 0. of mg/L, adenine 40 mg/Land GA3 0. 01 mg/L. A few calli derivedfrom protoplasts produced a root each after 13days of culture.