| 一种cDNA克隆的简易方法 |
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| 引用本文： | 余力,张迺蘅,张昌颖,刘惠珍,闵福源.一种cDNA克隆的简易方法[J].中国生物化学与分子生物学报,1988,4(4):358-363. |
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| 作者姓名： | 余力 张迺蘅 张昌颖 刘惠珍 闵福源 |
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| 作者单位： | 北京医科大学生化教研室
(余力，张迺蘅，张昌颖)，北京市肝炎研究所
(刘惠珍)，北京市肝炎研究所(闵福源) |
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| 摘 要： | 构建了一种适用于克隆cDNA的双分子质粒载体。使用此载体,以载体引物合成ds-cDNA后,用连接酶将cDNA-载体重组子自身环化,转化宿主菌。本文以此方法构建了人胚胎组织cDNA文库,转化菌中约50％含有cDNA插入片段。此方法大大简化了cDNA克隆步骤,提高了克隆效率。
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| 关 键 词： | 双分子质粒 载体-引物 人胚cDNA文库 |
| 收稿时间： | 1988-08-20 |
A SIMPLIFIED METHOD FOR cDNA CLONING |
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| Yu,Li Zhang,Nai-heng Liu,Hui-zhenMin,Hu-yuan Zhang,Chang-ying.A SIMPLIFIED METHOD FOR cDNA CLONING[J].Chinese Journal of Biochemistry and Molecular Biology,1988,4(4):358-363. |
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| Authors: | Yu Li Zhang Nai-heng Liu Hui-zhenMin Hu-yuan Zhang Chang-ying |
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| Institution: | (Department of Biochemistry, Beijing Medical University, Beijing) (Hepatitis Institute of Beijing, Beijing |
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| Abstract: | A dimer-vector for cDNA cloning was constructed. After the ds-cDNA was synthesied with the vectorprimer, the cDNA-vector recombinants were self ligated by T4 ligase and transformed into host cells. By the method a cDNA library of human embrorye was generated, and 50% of transformants contained the cDNA inserts. This method described here simplified significantly the cloning procedures of cDNA and increased the cloning efficiency. |
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| Keywords: | Dimer plasmid Vector-primer cDNA library |
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