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Heterochromatin restricts the mobility of nuclear bodies |
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| Authors: | Eugene?A?Arifulin Dmitry?V?Sorokin Anna?V?Tvorogova Margarita?A?Kurnaeva Yana?R?Musinova Oxana?A?Zhironkina Sergey?A?Golyshev Sergey?S?Abramchuk |
| Institution: | 1.Belozersky Institute of Physico-Chemical Biology,Lomonosov Moscow State University,Moscow,Russia;2.Laboratory of Mathematical Methods of Image Processing, Faculty of Computational Mathematics and Cybernetics,Lomonosov Moscow State University,Moscow,Russia;3.Koltzov Institute of Developmental Biology of Russian Academy of Sciences,Moscow,Russia;4.Faculty of Chemistry,Lomonosov Moscow State University,Moscow,Russia;5.LIA 1066 LFR2O French-Russian Joint Cancer Research Laboratory,Villejuif,France;6.UMR8126, CNRS, Institut de Cancérologie Gustave Roussy,Université Paris-Sud,Villejuif,France;7.Department of Cell Biology and Histology, Faculty of Biology,Lomonosov Moscow State University,Moscow,Russia |
| Abstract: | Nuclear bodies are relatively immobile organelles. Here, we investigated the mechanisms underlying their movement using experimentally induced interphase prenucleolar bodies (iPNBs). Most iPNBs demonstrated constrained diffusion, exhibiting infrequent fusions with other iPNBs and nucleoli. Fusion events were actin-independent and appeared to be the consequence of stochastic collisions between iPNBs. Most iPNBs were surrounded by condensed chromatin, while fusing iPNBs were usually found in a single heterochromatin-delimited compartment (“cage”). The experimentally induced over-condensation of chromatin significantly decreased the frequency of iPNB fusion. Thus, the data obtained indicate that the mobility of nuclear bodies is restricted by heterochromatin. |
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