A replication-time-controlling sequence element in <Emphasis Type="Italic">Schizosaccharomyces pombe</Emphasis> |
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Authors: | Vishnu?P?Tripathi Email author" target="_blank">Dharani?D?DubeyEmail author |
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Institution: | 1.Department of Biotechnology,Veer Bahadur Singh Purvanchal University,Jaunpur,India |
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Abstract: | Eukaryotic replication origins are highly variable in their activity and replication timing. The nature and role of cis-acting regulatory sequences that control chromosomal replication timing is not well defined. In the fission yeast, Schizosaccharomyces pombe, a 200-bp late-replication-enforcing element (LRE), has been shown to enforce late replication of ARS elements in plasmids. Here, we show that a short (133-bp) fragment of the LRE (shLRE) is required for causing late replication of adjoining origins in its native as well as in an ectopic early-replicating chromosomal location. Active from both sides of an early-replicating origin, the shLRE is a bona fide cis-acting regulatory element that imposes late replication timing in the chromosome. |
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