Relationship between genome packaging and Gag translation/AUG of primate lentiviruses |
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| Authors: | Sayuri Sakuragi Tatsuo Shioda Jun-ichi Sakuragi |
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| Institution: | Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, 565-0871, Japan |
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| Abstract: | About the relationship between retroviral genome packaging and translation, three possible modes (random-, trans-, and cis-) of packaging process could be assumed. In this report, we developed an assay system based on the RT-qPCR to measure the packaging efficiency of primate lentiviruses. With this system, we analyzed the genome packaging modes of primate lentiviruses such as HIV-1, 2, SIVmac and SIVagm. The data suggested that the modes of all viruses analyzed were very similar. In addition, we observed that the Gag-AUG sequences of them played important roles for maintaining efficient packaging, other than the initiation of translation. |
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| Keywords: | HIV SIV Lentivirus Packaging Genome dimerization Translation |
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