Changes in the composition of fatty acids and sterols of membrane lipids during induction and differentiation of Brassica napus (var. oleifera L.) callus |
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Authors: | Żur Iwona Skoczowski Andrzej Niemczyk Elżbieta Dubert Franciszek |
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Institution: | (1) Department of Plant Physiology Polish Academy of Sciences, The Franciszek Górski, Podłużna 3, 30-239 Kraków, Poland |
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Abstract: | Changes in the membrane lipid and sterols content and composition were studied during induction and differentiation in callus
cultures of Brassica napus var. oleifera. Callus induction was associated with an increase of DGDG content, significant changes in fatty acids composition of all
lipid fractions and increased degree of lipid unsaturation. The membrane lipid composition of tissue at different degrees
of differentiation was found to vary significantly, particularly two weeks after transfer of callus to regeneration medium.
The main differences concerned the content and composition of galactolipids. Curiously in many cases, these differences declined
during subsequent culture, in spite of the morphogenesis process which was in progress. Another result of differentiation
was the change in free sterol composition: in shoot regenerating calli the content of stigmasterol had rose whereas the accumulation
of campesterol decreased. Even though observed changes in membrane properties may not play a role in morphogenesis they are
nevertheless useful as developmental markers and can be invaluable in understanding biochemical basis of morphogenesis. |
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Keywords: | Brassica napus callus fatty acid composition galactolipids phospholipids oilseed rape regeneration ability sterols |
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