| 根田鼠下丘脑促性腺激素释放激素的放射免疫学测定 |
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| 引用本文： | 熊忠,杜继曾.根田鼠下丘脑促性腺激素释放激素的放射免疫学测定[J].兽类学报,2001,21(4):287-291. |
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| 作者姓名： | 熊忠 杜继曾 |
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| 作者单位： | 浙江大学生命科学学院 |
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| 摘 要： | 利用大鼠促性腺激素释放激素放射免疫学测定试剂检测了根田鼠下丘脑促性腺激素释放激素水平．测定线性范围为2.5 Pg至160pg/管，批内和批间差为1.7％ (n=10)和7.8%（n=4)，样品平均标准回收率为108.2±8.4％。根田鼠下丘脑促性腺激素释放激素测定为0.28±0.04 ng/mg湿重组织。从根田鼠下丘脑提取物稀释曲线与大鼠(人工合成)促性腺激素释放激素有较好的平行关系判断， 可以推知根田鼠下丘脑促性腺激素释放激素结构活性类似于大鼠(人工合成)的活性。
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| 关 键 词： | 促性腺激素释放激素 放射免疫学测定 根田鼠 下丘脑 |
| 文章编号： | 1000-1050(2001)04-0287-05 |
| 修稿时间： | 2000年10月8日 |
RADIOIMMUNOASSAY OF HYPOTHALAMIC GONADOTROPIN-RELEASING HORMONE CONTENTS OF ROOT VOLE(MICROTUS OECONOMUS) |
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| XIONG Zhong,DU Jizeng.RADIOIMMUNOASSAY OF HYPOTHALAMIC GONADOTROPIN-RELEASING HORMONE CONTENTS OF ROOT VOLE(MICROTUS OECONOMUS)[J].Acta Theriologica Sinica,2001,21(4):287-291. |
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| Authors: | XIONG Zhong DU Jizeng |
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| Abstract: | The contents of the hypothalamic gonadotrophin-releasing hormone (GnRH) of the root vole (Microtus oeconomus) were successfully tested by using a rat GnRH and its antiserum with a sensitivity range of 2.5 pg/tube. Intra and inter assay coefficient of variation were 1.7%(n=10) and 7.8%(n=4), respectively. The extract of hypothalamus of the vole showed a good parallel dose response curve with synthetic rat GnRH. The rate of average standard recovery was 108.2±8.4%. The mean levels of hypothalamus of the vole contains a GnRH similar to rat GnRH. |
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| Keywords: | Gonadotrophin releasing hormone Radioimmunoassay Root vole ( Microtus oeconomus ) Hypothalamus |
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