PilJ Localizes to Cell Poles and Is Required for Type IV Pilus Extension in Pseudomonas aeruginosa |
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Authors: | Paul A. DeLange Tracy L. Collins George E. Pierce Jayne B. Robinson |
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Affiliation: | (1) Biology Department, University of Dayton, 300 College Park, Dayton, OH 45469-2320, USA;(2) Department of Biology, Georgia State University, P.O. Box 4010, Atlanta, GA 30302-4010, USA;(3) Biology Department, University of Dayton, 300 College Park, Dayton, OH 45469-2320, USA |
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Abstract: | Twitching motility allows Pseudomonas aeruginosa to respond to stimuli by extending and retracting its type IV pili (TFP). PilJ is a protein necessary for this surface-associated twitching motility and bears high sequence identity with Escherichia coli methyl-accepting chemotaxis proteins (MCP). Here, we report that whereas wild-type P. aeruginosa PAO1 cells have extended pili at a single pole, pilJ mutant cells have shortened pili often at both poles despite normal levels of pilin accumulation, suggesting that PilJ is required for full TFP assembly/extension. Using yellow fluorescent protein fusions (pilJ-yfp), both plasmid born and in-frame chromosomal constructs, we determined that PilJ localizes to both poles of the cell. Overexpression of pilJ-yfp resulted in the protein accumulating between the poles. Paul DeLange and Tracy Collins contributed equally to this work. |
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Keywords: | Twitching motility PilJ Type IV pili Pseudomonas |
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