Identification of enolase as a plasminogen-binding protein in excretory-secretory products of Fasciola hepatica |
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Authors: | Bernal Dolores de la Rubia Jose Enrique Carrasco-Abad Ana M Toledo Rafael Mas-Coma Santiago Marcilla Antonio |
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Affiliation: | Departament de Bioquímica y Biología Molecular, Facultat de Ciències, Universitat de Valencia, C/Dr. Moliner, 50, 46100 Burjassot, Valencia, Spain. |
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Abstract: | We have followed a combined proteomic approach to identify proteins of Fasciola hepatica that could be involved in host-parasite interactions. Using two-dimensional gel electrophoresis, far Western immunoblot and mass spectrometry analyses, we have identified the enolase enzyme, present in the excretory/secretory materials of F. hepatica, as a human plasminogen-binding protein. This enzyme has an apparent molecular weight of 47 kDa with pI ranging from 6.2 to 7.2. These results suggest that enolase could act as a plasminogen receptor. |
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