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急纤虫营养细胞和休眠细胞的中间纤维-核骨架体系
引用本文:李艺松,隋淑光,张莉,顾福康. 急纤虫营养细胞和休眠细胞的中间纤维-核骨架体系[J]. 动物学研究, 2004, 25(2): 147-152
作者姓名:李艺松  隋淑光  张莉  顾福康
作者单位:华东师范大学,生命科学学院,上海,200062
基金项目:国家自然科学基金资助项目 (3 0 2 70 160 ),高等学校博士学科点专项科研基金资助
摘    要:利用生化分级抽提、DGD包埋—去包埋透射电镜术和SDS—PAGE凝胶电泳,研究了膜状急纤虫营养细胞和休眠细胞内中间纤维—核骨架体系的分化特征及其蛋白组成。观察到营养细胞中,位于细胞质不同区域的中间纤维形成网状,其网络的密度不同;核骨架中,核纤层位于细胞核周缘,薄层状,厚约50nm;核内骨架由较致密的纤维网络组成。休眠细胞内该结构体系依然存在,但位于细胞内不同层次的纤维网比营养细胞的同种结构要致密得多,这可能与纤毛虫脱分化时细胞大范围的收缩有关;休眠细胞的包囊壁中层壁存在相当于中间纤维的网络结构。SDS—PAGE电泳图谱显示,休眠细胞内该体系的蛋白组成发生了较明显的变化,其中保留了营养细胞的部分蛋白条带,丢失了部分条带,同时还产生了一些特异性条带。分析表明,膜状急纤虫的中间纤维—核骨架体系是细胞在营养条件下和休眠状态下都稳定存在的结构;而纤毛虫形成休眠细胞后中间纤维—核骨架体系及蛋白组成上的变化提示,细胞在休眠状态下,基因的表达水平与营养细胞是不同的。

关 键 词:急纤虫 营养细胞 休眠细胞 中间纤维-核骨架体系 基因 SDS-PAGE
文章编号:0254-5853(2004)02-0147-06

Intermediate Filament-Nuclear Matrix System of Vegetative- and Resting-Cell of Tachysoma pellionella (Ciliophora,Protozoa)
LI Yi song,SUI Shu guang,ZHANG Li,GU Fu kang. Intermediate Filament-Nuclear Matrix System of Vegetative- and Resting-Cell of Tachysoma pellionella (Ciliophora,Protozoa)[J]. Zoological Research, 2004, 25(2): 147-152
Authors:LI Yi song  SUI Shu guang  ZHANG Li  GU Fu kang
Affiliation:Department of Biology,East China Normal University,Shanghai 200062,China
Abstract:Biochemical characters and protein compositions of intermediate filament (IF) nuclear matrix (NM) system of vegetative and resting cell of Tachysoma pellionella were studied by the method of serial selective extraction,diethylene glycol distearate (DGD) embedment and emebedment free electron microscopy,and SDS PAGE.The result shows that in the vegetative cell,the IF,with diverse density in different regions,occurs in the cytoplasm;50?nm thick and thin layer shaped lamina,in the periphery of nucleus;the NM is composed of a more densely arranged filament network.The same kind of network also exists in the resting cell,as well as in the middle level layer of cyst wall,but presents more compact compared with the similar structure of vegetative cell,and this phenomenon may refer to the comprehensive contraction in the course of encystment.The result of SDS PAGE shows that protein composition of this system has changed notably when the cyst is formed,of which,part of the proteins have been preserved and part lost,and some new and distinct proteins occur.The following conclusions can be obtained:the IF NM system is a stable structure in both vegetative and resting cell;changes of IF NM system and protein composition after encystment reveal that gene expression of resting cell is different from that of vegetative cell.
Keywords:Tachysoma pellionella  Vegetative cell  Resting cell  Intermediate type filament nuclear matrix
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