外源DNA导入在蔬菜分子育种中的研究进展

随着转基因方法的多样化和技术体系的不断完善,转基因技术在蔬菜育种上的应用取得了显著成果,成为蔬菜种质创新和品种改良的有效途径。介绍了几种不依赖组织培养的外源DNA导入技术,包括花粉管通道法、农杆菌浸渍法、激光微束法、PEG法、电击法和脂质体法,从育种角度比较了这些方法的优缺点,并对其在蔬菜育种中的应用现状、存在问题及应用前景进行了较为全面的综述。

Progress of Research on Exogenous DNA Introduction in Vegetable Breeding

6 physical methods of exogenous DNA introduction,including pollen-tube pathway,agrobacterium infiltration,laser microbeam-mediated transformation,PEG-mediated transformation,electroporation-mediated transformation and liposome-mediated transformation were introduced in this article,and their advantages and disadvantages were compared in detail from the view of vegetable breeding.The application,existing problems and prospect of exogenous DNA introduction were systematically summarized.