glo-3, a novel Caenorhabditis elegans gene, is required for lysosome-related organelle biogenesis |
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| Authors: | Rabbitts Beverley M Ciotti Marcela K Miller Natalie E Kramer Maxwell Lawrenson Andrea L Levitte Steven Kremer Susan Kwan Elizabeth Weis Allison M Hermann Greg J |
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| Institution: | Department of Biology, Lewis & Clark College, Portland, Oregon 97219, USA. |
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| Abstract: | Gut granules are specialized lysosome-related organelles that act as sites of fat storage in Caenorhabditis elegans intestinal cells. We identified mutations in a gene, glo-3, that functions in the formation of embryonic gut granules. Some glo-3(−) alleles displayed a complete loss of embryonic gut granules, while other glo-3(−) alleles had reduced numbers of gut granules. A subset of glo-3 alleles led to mislocalization of gut granule contents into the intestinal lumen, consistent with a defect in intracellular trafficking. glo-3(−) embryos lacking gut granules developed into adults containing gut granules, indicating that glo-3(+) function may be differentially required during development. We find that glo-3(+) acts in parallel with or downstream of the AP-3 complex and the PGP-2 ABC transporter in gut granule biogenesis. glo-3 encodes a predicted membrane-associated protein that lacks obvious sequence homologs outside of nematodes. glo-3 expression initiates in embryonic intestinal precursors and persists almost exclusively in intestinal cells through adulthood. GLO-3 | |
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