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Activation of Dictyostelium discoideum guanylate cyclase by ATP. |
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| Authors: | J M Mato |
| Affiliation: | 1. Department of Occupational Diseases, National Institute of Industrial Health, Nagao, Tama-ku, Kawasaki 213, Japan;2. Department of Experimental Toxicology, National Institute of Industrial Health, Nagao, Tama-ku, Kawasaki 213, Japan |
| Abstract: | Human leucocyte cell cultures were stimulated to initiate DNA synthesis by phytohemagglutinin and mercuric chloride. Both mitogens enhanced the accumulation of β2-microglobulin in the medium, which was synthesized by lymphocytes. Mercuric chloride promoted the accumulation of this protein optimaly with a concentration (1 × 10?5M) to produce the maximum stimulation of DNA synthesis. Combined use of phytohemagglutinin (50 μg/ml) and mercuric chloride (1 × 10?5M) produced additive effect on both DNA synthesis and β2-microglobulin accumulation. These findings suggest that mercuric ion causes the proliferative response of lymphocytes by a mechanism different from that for the stimulation by phytohemagglutinin. |
| Keywords: | Present address: Faculty of Pharmaceutical Sciences Teikyo University Sagamiko Kanagawa 199-01 Japan. To whom all correspondence should be addressed. |
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