UP-PCR Genotyping and rDNA Analysis of Ascochyta pisi Lib |
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| Authors: | P S Lübeck I A Alekhina M Lubeck S A Bulat |
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| Institution: | Plant Pathology Section, Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsensnej 40, DK-1871 frederiksherg C. Denmark: Petersburg Nuclear Physics Institute (PNPI). Department of Molecular and Radiation Biophysics, Gatchina 188350, Russia |
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| Abstract: | Isolates of Ascochyta pisi, previously identified as separate pathotypes, were genotyped by rDNA-RFLP and UP-PCR using 8 UP-primers and 2 arbitrary primers individually or in pair-wise combinations. The appearance of polymorphic UP-PCR products for all studied isolates facilitated their differentiation. The markers were suitable in identifying isolates, and may be useful for developing isolate- or pathotype-specific PCR-based diagnostic assays. In studying genetic relatedness by UP-PCR among A. pisi isolates, two distinct clusters were revealed. rDNA analysis of the isolates based on endo-ntjclease digestion of amplified ITSl and 1TS2-25S nuclear rDNA regions did not separate the isolates, whereas the variabihty of the isolates using UP-PCR was more than 40%. |
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