Degradation 1,2-dimethylbenzene by Corynebacterium strain C125 |
| |
| Authors: | Gosse Schraa Brigit M Bethe Alex R W Van Neerven Will J J Van Den Tweel Ewout Van Der Wende Alexander J B Zehnder |
| |
| Institution: | (1) Department of Microbiology, Agricultural University, 6703 CT Wageningen, The Netherlands;(2) Present address: Department of Chemical and Civil Engineering, Montana State University, 59717 Bozeman, MT, USA |
| |
| Abstract: | In an attempt to obtain bacteria growing on 1,2-dimethylbenzene as sole carbon and energy source two different strains were isolated. One was identified as an Arthrobacter strain, the other as a Corynebacterium strain. Corynebacterium strain C125 was further investigated. The organism was not capable to grow on 1,3- and 1,4-dimethylbenzene. cis-1,2-Dihydroxycyclohexa-3,5-diene oxidoreductase and 3,4-dimethylcatechol-2,3-dioxygenase activity was found in cell extracts. When 3,4-dimethylcatechol was added to cell extract of 1,2-dimethylbenzene-grown cells, first a compound with the spectral properties of 2-hydroxy-5-methyl-6-oxo-2,4-heptadienoate was formed and subsequently acetate was produced. It is proposed that dioxygenases are involved in the initial steps of 1,2-dimethylbenzene degradation, and ring opening proceeds via meta-cleavage. |
| |
| Keywords: | 1 2-dimethylbenzene ortho-xylene degradation of methyl substituted aromatic compounds Corynebacterium |
| 本文献已被 SpringerLink 等数据库收录！ |
|
