Micropropagation of Lepidium virginicum (Brassicaceae), a plant with antiprotozoal activity |
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Authors: | Lidia Osuna María Esther Tapia-Pérez Odette Figueroa Enrique Jiménez-Ferrer María Luisa Garduño-Ramírez María Teresa González-Garza Pilar Carranza-Rosales Delia Elva Cruz-Vega |
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Institution: | 1. Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina 1, Col. Centro, C.P. 62790, Xochitepec, Morelos, México 2. Facultad de Farmacia, Departamento de Fisiología Vegetal, Universidad de Barcelona, Barcelona, Espa?a 3. Facultad de Medicina, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México 4. Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México 5. Centro de Investigación y Extensión en Ciencias de la Salud, Instituto Tecnológico de Estudios Superiores de Monterrey, Monterrey, Nuevo León, México 6. División de Biología Celular y Molecular, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Monterrey, Nuevo León, México
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Abstract: | Summary Micropropagation is a technique to ensure a constant and uniform source of medicinal plants. In this report, we describe the
micropropagation of Lepidium virginicum L. (Brassicaceae), a wild plant used as an antiamoebic in traditional Mexican medicine. In vitro-germinated seeds were cultured in Murashige and Skoog (MS) medium to obtain pathogen-free cotyledons, hypocotyls, and apical
bud (AB) explants. For induction of morphogenesis, the effect of cytokinins, benzyladenine (BA) and kinetin (KN), combined
with auxin, indole-3-acetic acid (IAA) was evaluated. The best rate of shoot proliferation was induced 15 d after culture
on MS mineral medium supplemented with IAA∶KN (0.57∶13.94 μM) from AB explants. Maximum shoot elongation was achieved without plant growth regulators. The effect of indole-3-butyric
acid (IBA) (14.76 μM) was evaluated for in vitro root induction; 60 d after culture all the shoots had developed roots. All rooted plants were successfully transferred to
pots and 100% acclimatized in ex vitro conditions. The methanol extracts from the micropropagated active explants of L. virginicum showed and IC50 antiprotozoal value between 141.90 and 268.53 μg ml−1. |
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Keywords: | acclimatization antiprotozoal activity Lepidium virginicum micropropagation |
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